Adaptation and Study of AIDS Viruses in Animal and Cell Culture Systems
Abstract
Several small animal models of HIV infection have been proposed. We have developed a means to support the replication of HIV-1 which can produce large populations of infected mice for therapy screening and host-virus interactions. Nude mice (3-4 wks old) were exposed to 600 R's of 137C's irradiation and inoculated with 1 x 107 HIV-1 infected CEM cells. The animals were followed daily for weight gain and tumor progression. Groups of 6 animals were exsanguinated at 8 intervals over a 9 week period and necropsies were performed. Plasma p24 antigen was detected at day 3 and rose over 9 weeks (2200 pg/ml). Where possible, plasma antigen was neutralized with human antisera to HIV-1. The infected mice did not exhibit any weight loss, but a highly significant difference was seen in tumor progression when compared to controls. Using immunohistochemistry, sheep raised polyclonal antibodies to viral p24 and gp 120 detected HIV proteins within tumor cells and mouse splenic macrophages. (JES)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 30, 1989
- Accession Number
- ADA221651
Entities
People
- Neal T. Wetherall
Organizations
- Vanderbilt University