Cloning, Characterization, and Expression of Animal Toxin Genes for Vaccine Development
Abstract
Gene libraries have been constructed from the messenger ribonucleic acid (mRNA) isolated from venom glands of different poisonous animals such as snakes, scorpions, and snails. The gene banks thus created contain recombinant clones harboring DNA sequences encoding toxins with various pharmacological activities, ranging from myonecrosis-inducing to those affecting neuronal transmission. A number of these clones have been isolated and characterized, and gene expression has been attempted with limited success in Escherichia coli, baculovirus, and in two malian cell expression systems by using either cDNAs or synthetically constructed genes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 27, 1990
- Accession Number
- ADA221849
Entities
People
- Leonard A. Smith
Organizations
- United States Army Medical Research Institute of Infectious Diseases