AFRRI Reports: January-March 1990
Abstract
Aqueous solutions of calf thymus deoxyribonucleic acid ( DNA) were exposed to hydrogen peroxide in the presence of air. Base products formed in DNA were identified and quantitated following acid hydrolysis and trimethylsilylation using gas chromatography-mass spectrometry. The yields of these products were dependent upon the hydrogen peroxide concentration, and increased in the following order: 8-hydroxyadenine, cytosine glycol, and 2,6- diamino-4-hydroxy-5-formamidopyrimidine, 8-hydroxyguanine, thymine glycol, and 4,6-diamino-5-formamidopyrimidine. Previous studies have shown that these compounds are typically formed in DNA in aqueous solution by hydroxyl radical generated by ionizing radiation. Hydrogen peroxide is thought to participate in a Fenton-like reaction with transition metals, which are readily bound to DNA in trace quantities, resulting in the production of hydroxyl radicals close to the DNA.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1990
- Accession Number
- ADA221905
Entities
Organizations
- Armed Forces Radiobiology Research Institute