Coxiella Burnetti Vaccine Development: Lipopolysaccharide Structural Analysis

Abstract

This Midterm report details new analytical approaches developed for the determination of endotoxin structures at much improved sensitivity and specificity, and an application of those techniques to Coxiella burnetti LPS. Foremost in this methodology is a reducing-end reagent synthesized to provide femtomole (10-15) detection for 0-antigen oligosaccharides. A second development focuses on methodology to extract the details of linkage, branching, and sequence, by periodate oxidation, supercritical fluid chromatography, and mass spectrometry. These techniques combine to provide the elements of a global approach to oligosaccharide structure. The utility of supercritical fluid chromatography for a determination of Lipid-A from Salmonella minnesota has been demonstrated. Finally, the structural features of Lipid-A from Coxiella burnetii is described using a combination of these newly developed analytical techniques. Keywords: Lipopolysaccharide; Immunogen; Coxiella burnetii.

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Document Details

Document Type
Technical Report
Publication Date
Dec 29, 1989
Accession Number
ADA222505

Entities

People

  • V. N. Reinhold

Organizations

  • Harvard T.H. Chan School of Public Health

Tags

Communities of Interest

  • Biomedical
  • Space

DTIC Thesaurus Topics

  • Acetic Acid
  • Alcohols
  • Amino Sugars
  • Anhydrides
  • Carbohydrates
  • Chemical Synthesis
  • Chemistry
  • Complex Mixtures
  • Detection
  • Elements
  • Fatty Acids
  • Lipopolysaccharides
  • Mass Spectrometry
  • Organic Chemistry
  • Spectrometry
  • Structural Analysis
  • United States

Readers

  • Immunology
  • Organic Chemistry
  • Systems Analysis and Design

Technology Areas

  • Biotechnology