Mass-Screening of Curarimimetic Neurotoxin Antagonists

Abstract

The third year of the contract period was devoted to testing several ion flux protocols for the distinction between toxic and therapeutic blockers of curarimimetric neurotoxins. First a cell line suitable for acetylcholine receptor channel analysis was identified; among several rodent and human lines the murine tumor line BC3-H1 was selected because of its high receptor content. After determining optimal culture conditions for this line attempts were made to miniaturize the flux assay; we were able to perform 86Rb flux measurement in standard 24-well multititer plates. Keywords: Neurotoxin; Acetylcholine receptor; Ion flux; Antidote.

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Document Details

Document Type
Technical Report
Publication Date
Mar 10, 1990
Accession Number
ADA222729

Entities

People

  • Jakob Schmidt

Organizations

  • State University of New York

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  • Biomedical

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  • Abstracts
  • Antidotes
  • Bibliographies
  • Biomedical Research
  • Cell Line
  • Cells
  • Classification
  • Contracts
  • Culture Techniques
  • Incubation
  • Maryland
  • Measurement
  • Muscle Cells
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  • New York
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  • Molecular and Cellular Biochemistry
  • Oncology (Cancer Research).
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