Reciprocal Expression of Human ETS1 and ETS2 Genes during T-Cell Activation: Regulatory Role for the Protooncogene ETS1

Abstract

The expression of the protooncogenes ETS1 and ETS2 has been studied in purified human T cells activated either by cross-linking of the T-cell receptor-CD3 complex on their cell surface or by direct stimulation with phorbol esters and ionomycin. Our results show that resting T cells express high levels of ETS1 mRNA and protein, while expression of ETS2 is undetectable. Upon T-cell activation, ETS2 mRNA and proteins are induced, while ETS1 gene expression decreases to very low levels. Late after stimulation, ETS1 mRNA is reinduced and maintained at a high level, while ETS2 gene expression decreases to undetectable levels. Therefore, it appears that in human T cells, ETS2 gene products are associated with cellular activation and proliferation, while ETS1 gene products are preferentially expressed in a quiescent state. Keywords: Reprints, Genetic studies, Human ETS1 and ETS2 genes, T-cell activation, Protooncogene ETS1, Regulatory role, C-ets gene family, Cell proliferation.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1990
Accession Number
ADA222964

Entities

People

  • Carl H. June
  • Craig. B. Thompson
  • Narayan K. Bhat
  • Shigeyoshi Fujiwara
  • Tullia Lindsten

Organizations

  • Naval Medical Research Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Biology
  • Blood
  • Cell Biology
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Classification
  • Fibroblasts
  • Gene Expression
  • Lymphocytes
  • New York
  • Notation
  • Proteins
  • Standards
  • T Lymphocytes
  • Thymocytes

Fields of Study

  • Biology

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Immunology
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology