Preparation and Characterization of Mouse and Human Monoclonal Antibodies to Botulinum Toxins

Abstract

The goal during this year of the project was to begin preparing and characterizing mouse and human monoclonal antibodies to botulinum toxoids. The initial step was to refine enzyme immunoassays for identifying both mouse and human monoclonal antibodies in hybridoma culture supernatants. Progress in this area has been good, though minor technical problems remain to be solved. Optimization of these assays was accomplished with hyperimmune mouse and human antisera obtained from Dr. Martin Crumrine, USAMRIID. Keywords: Mice, Human monoclonal antibodies, Enzyme immunoassays, Type B toxoids, Type E toxoids, Toxic, Microbiology, Medical research, Botulinum toxins.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1983
Accession Number
ADA223009

Entities

People

  • Gerald W. Fisher
  • Kenneth W. Hunter
  • Val G. Hemming

Organizations

  • Uniformed Services University of the Health Sciences

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical And Dental Materials
  • Biomedical Research
  • Blood
  • Cells
  • Clone Cells
  • Coatings
  • Contracts
  • Dielectric Polymers
  • Dilution
  • Immune Serums
  • Immunoassay
  • Laboratory Animals
  • Molecules
  • Polymeric Films
  • Public Health
  • Titration

Fields of Study

  • Biology

Readers

  • Immunology
  • Microbial Pathology
  • Technical Research and Report Writing.