Systemic Activation of the IFN System by Exposure of Natural Epithelia to Physiologic (Low Dose) Levels of IFN
Abstract
Splenocytes treated with very low concentrations of cross-reactive recombinant human interferon alpha A/D (IFN A/D) provided significant protection in mice against the effects of Semliki Forest virus (SFV). In addition, groups of mice inoculated intranasally with splenocytes treated with 30 IU/ml of IFN showed greater protection against encephalitis and death from SFV than did those inoculated with 100 IU/ml of IFN-treated splenocytes. Thus the response was biphasic. In 13 experiments mice treated with low concentrations of IFN A/D or mouse IFN/alpha/Beta in their drinking water were protected from SFV (p<0.0005). The responses occurred over a narrow concentration range and were biphasic. Thus higher concentrations of IFN were not protective. Duplication of the biphasic effects by recombinant human IFN strongly suggests they were activated by the IFN.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 20, 1990
- Accession Number
- ADA223410
Entities
People
- G. J. Stanton
- Thomas K. Hughes
Organizations
- University of Texas Medical Branch