Separation of T and B Lymphocytes with Magnetic Beads
Abstract
Techniques have been established using immunomagnetic beads for the positive selection (target cells rosetted by the beads) of lymphocyte subpopulations for use in HLA typing (Vartdal et al., 1986). While these techniques provide for rapid isolation and a high degree of purity, only lymphocytotoxicity techniques which employ immunofluorescence for HLA typing can be used with cells isolated this way. By modifying these techniques to employ negative selection (target cells left in suspension, unwanted cells rosetted by the beads), the purity and speed of the technique are preserved while the immunofluorescence requirement is eliminated. The technique can be applied for the isolation of either B or T cells, depending upon the choice of monoclonal antibody for bead coating.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1990
- Accession Number
- ADA223528
Entities
People
- Julia S. Heard
- Nancy F. Hensel
Organizations
- Naval Medical Research Center