Isolating and Characterizing Genes of Liver Stage Antigens with Vaccine Potential

Abstract

A 175kd Plasmodium falciparum blood stage antigen with binds to erythrocytes and to merozoites, designated EBA-175, appears to be involved in the initial attachment of the merozoite to the red cell, and it has been postulated that antibodies to EBA could inhibit merozoite reinvasion. The gene encoding EBA-175 has been isolated, cloned and sequenced by using monospecific antibodies selected from malaria immune monkey serum by affinity purification with EBA-175 adsorbed to nitrocellulose. Peptides predictive of antigenic determinants were synthesised, conjugated to KLH and used to immunise mice and rabbits. Antibodies raised against peptide KIM4 recognized authentic EBA-175 in immunoblots, immunoprecipitated EBA-175 and reacted with free merozoites and segmenters. Keywords: Vaccine development; RA 1; Malaria; Non-Circumsporozoite antibodies; Gene libraries.

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Document Details

Document Type
Technical Report
Publication Date
Feb 14, 1990
Accession Number
ADA223617

Entities

People

  • Betty K. Sim

Organizations

  • Johns Hopkins University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amino Acids
  • Blood
  • Cells
  • Chemistry
  • Classification
  • Genetic Structures
  • Infectious Diseases
  • Laboratory Animals
  • Malaria
  • Materials
  • Notation
  • Polymerase Chain Reaction
  • Security
  • Sialic Acids
  • Spores
  • Symbols
  • Vaccines

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Parasitology and Pharmacology of Malaria.

Technology Areas

  • Biotechnology