Pathophysiology and Toxicokinetic Studies of Blue-Green Algae Intoxication in the Swine Model
Abstract
An attempt by New England Nuclear to produce radiolabelled MCLR involved synthetic labelling using tritiated water and Rh/Al2O3. Chromatography and mass spectrometry revealed radiolabel incorporation into both the toxin and contaminants. The nonspecifically tritiated toxin had a specific activity of 05 uCi/mg. Subsequently, a method recommended by our laboratory utilizing tritiated water with pyridine was performed by New England Nuclear; however, decomposition of the toxin occurred under the conditions used. MCLR was highly bound when added to 'super-activated' charcoal at 1:100. When given to rats via an ileal loop shortly after toxin administration, 'superchar' settled out, but was partially effective in limiting MCLR absorption (as indicated by increases in liver weight). Keywords: Blue-green algae, Phycotoxin, Microcystin, Cyanoginosin anatoxin-a(s), Pathophysiology, Radiolabelled toxin, Analysis, RA 1, Anatoxin-a.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 26, 1989
- Accession Number
- ADA223638
Entities
People
- Andrew M. Dahlem
- Val R. Beasley
- Wanda M. Haschek-hock
- Wayne W. Carmichael
- William O. Cook
Organizations
- University of Illinois Urbana–Champaign