Flow Cytometric Analysis of Hepatocytes from Normal, PFDA, and PH/DEN/ PB-Treated Rats

Abstract

The goal of this project was to provide preliminary data regarding the hepatotoxic and immunotoxic effects of perfluorodecanoic acid (PFDA). Flow cytometric evaluation of hepatocytes from PFDA-treated rats revealed an increase in size and granularity and a shift from tetraploidy towards diploidy, in rats treated with 50 mg/kg PFDA, as well as a suggestion of interference with lymphocyte blast transformation of splenocytes in these rats. These preliminary studies suggest that PFDA induces hepatocellular alterations which are detectable by flow cytometry, and induces both morphologic and possibly functional modulation of the immune system. Further studies are indicated to determine the significance of these findings. Keywords: Perfluorodecanoic ACID(PFDA); Hepatocarcinogenesis; Preneoplastic lesions; Flow cytometry; Immunotoxicity.

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Document Details

Document Type
Technical Report
Publication Date
Dec 31, 1989
Accession Number
ADA223765

Entities

People

  • Donald E. Frazier
  • Melinda J. Tarr

Organizations

  • Ohio State University

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Air Force
  • Air Force Facilities
  • Antibodies
  • B Lymphocytes
  • Biological Sciences
  • Cells
  • Chemistry
  • Classification
  • Drug Abuse
  • Immune System
  • Lymphatic System
  • Lymphocytes
  • Medical Personnel
  • Security
  • Universities

Fields of Study

  • Biology

Readers

  • Immunology
  • Toxicology/Environmental Toxicology