Chemical Blistering: Cellular and Macromolecular Components
Abstract
The overall objective of this investigation is to elucidate in culture the molecular mechanisms by which bis-(beta-chlorethyl) sulfide (BCES) exerts its vesicant action when applied topically to human skin. The technical objectives of the project are to establish morphological, cytochemical and/or biochemical indicators of epidermal mustard toxicity and to examine and evaluate the relevance of selected parameters to vesication. Three biological systems used in this study of them molecular and cellular effects of exposure to BCES are (a) the stratified terminally differentiating primary culture of cutaneous keratinocytes derived from human or rat skin grown on a collagen gel or nylon membrane as substratum positioned at the air-medium interface; (b) the submerged monolayer of proliferating and early differentiated keratinocytes grown on the plastic surface of the Petri dish, and (c) purified populations of basal cells, which are obtained by sedimentation in a density gradient and are used as innocula for cell culture. Keywords: Mustard, Kerotinocyte, Tissue culture, Aklylation, RA5, Toxicity, Chemical blistering, Mitochondria, Metabolism, DNA repair, Epidermal proliferation, Epidermal differentiation, Vesicants, BCES, Bis-(beta-chloroethyl) sulfide.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 30, 1987
- Accession Number
- ADA223808
Entities
People
- I. A. Bernstein
Organizations
- University of Michigan