Cloning and Expression of Genes for Dengue Virus Type-2 Encoded-Antigens for Rapid Diagnosis and Vaccine Development
Abstract
Using a prokaryotic expression system, it was possible to map the antigenic determinant involved in neutralization of 3H5 monoclonal antibody. A synthetic peptide containing the epitope elicits good neutralization antibodies with high titer in rabbits. Using a novel strategy, it was possible to produce truncated RNA molecules containing various lengths of 3'-terminal sequences of DEN-2 RNA with the same polarity and nucleotide sequence. The in vitro synthesized RNA could be labeled to a high specific activity with P-32. Using radiolabeled RNA molecules, we could demonstrate the formation of a stable secondary structure containing the 3'-terminal sequences. Keywords: Epitope mapping; Synthetic peptide; Anti-peptide antibody; Neutralization epitope; Secondary structure of viral genome.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 11, 1989
- Accession Number
- ADA223891
Entities
People
- Radha K. Padmanabhan
Organizations
- University of Kansas Medical Center