Monoclonal Antibodies for Dengue Virus prM Glycoprotein Protect Mice against Lethal Dengue Infection
Abstract
Five murine monoclonal antibodies (Mabs) reactive against the prM glyco-proteins of DEN-3 and -4 were used to passively protect mice in vivo against lethal challenge with homologous and heterologous dengue virus serotypes. Four of the 5 prM-reactive monoclonals cross-protected mice against heterologous challenge, whereas 1 protected against challenge with only the homologous serotype. Although in vitro binding to virions was readily demonstrated, only 2 of the prM Mabs had detectable neutralizing activity. The neutralizing activity could not be enhanced by anti-mouse immunoglobulin or complement. However, 4 of the 5 prM Mabs fixed complement. This is the first report of prM-specific Mabs that are protective in mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 15, 1989
- Accession Number
- ADA223968
Entities
People
- B. M. Kaufman
- D. R. Dubois
- M. K. Gentry
- P. L. Summers
- W. H. Cohen
Organizations
- Walter Reed Army Institute of Research