Differences in Conformational Stability Between Native and Phosphorylated Acetycholinesterase as Evidenced by a Monoclonal Antibody

Abstract

Monoclonal antibody 25B1 generated against diisopropyl phosphorofluoridate-inhibited fetal bovine serum acetylcholinesterase has been extensively characterized with respect to its anticholinesterase properties. This antibody demonstrated considerably different properties from previously reported inhibitory antibodies raised against acetylcholinesterase in terms of the degree of inhibition (98%), the high degree of specificity, and the stability of the antigen-antibody complex. Keywords: Acetylcholinesterase, Monoclonal antibodies, Organophosphates.

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Document Details

Document Type
Technical Report
Publication Date
Mar 13, 1990
Accession Number
ADA224018

Entities

People

  • Bhupendra P. Doctor
  • Mary K. Gentry
  • Yacov Ashani

Organizations

  • Walter Reed Army Institute of Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Acetylcholinesterases
  • Biochemistry
  • Blood
  • Chemical Synthesis
  • Chemistry
  • Crystal Structure
  • Dissociation
  • Emission Spectra
  • Enzyme Inhibitors
  • Equations
  • Fluorescence
  • Inhibition
  • Inhibitors
  • Kinetics
  • Monitoring
  • Spectroscopy

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Neurotoxicology