Immunotoxicological Assays Using the Japanese Medaka

Abstract

Phagocytic blood cells secrete reactive oxygen intermediates (ROI) which are involved in immune defense reactions such as killing pathogenic microorganisms. The long-term goal of this project is to determine the effects of sublethal exposure to xenobiotics on ROI, and other immunological parameters, using a small aquarium fish model (the Japanese medaka). A relevant blood cell model system (Crassos trea virginica) was used to develop lap assays for key ROI parameters (superoxide anion, hydrogen peroxide, singlet oxygen, etc.). In addition to medaka studies, this was the first characterization of ROI in this species (oyster). The application of ROI assays to fish (goldfish, medaka, Fundulus) produced encouraging preliminary results. Exposure of C. virginica blood cells to an environmental pollutant (cadmium) produced ROI inhibition, indicative of immunosuppression. The effects of in vivo and in vitro exposure of medaka macrophages to xenobiotics on ROI are under study. (JES)

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Document Details

Document Type
Technical Report
Publication Date
May 14, 1990
Accession Number
ADA224420

Entities

People

  • Robert S. Anderson

Organizations

  • University of Maryland

Tags

DTIC Thesaurus Topics

  • Animals
  • Anti-Infective Agents
  • Azo Compounds
  • Blood
  • Blood Cells
  • Cells
  • Chemistry
  • Classification
  • Fungi
  • Hemocytes
  • Hydrogen
  • Indicator Dyes
  • Laboratory Animals
  • Macrophages
  • Microorganisms
  • Oxygen
  • Rocket Oxidizers

Readers

  • Aquatic Ecology
  • Immunology
  • Life Cycle Cost Analysis