Drug Retention during Animal Inhalation Exposure by FT-IR Spectroscopy
Abstract
A unique analytical method for the determination of the amount of volatile drug retention by animals during inhalation exposure experiments was developed and evaluated. A large-volume collection system was used to collect and store all exhaled air from the animal. The collected air was analyzed by FT- IR spectroscopy utilizing a Nicolet 5SXB spectrometer and a Spectra-Tech, Inc. 10 meter long-path gas cell. The developed technique was evaluated using bis-(trifluoromethyl) disulfide (TFD) administered to adult sheep. Control experiments confirmed the system's ability to capture 100% of the administered compound. It was observed that the sheep retained an average of 4.4 mg of this compound per Kg of body weight during a 10-min exposure period at an air concentration of 5.0 mg per liter. After quantitative analysis, compounds exhaled by the animal were concentrated by passing the collected air through a series of dry ice and liquid nitrogen cooled traps. FT-IR analysis of the collected concentrated products indicated that TFD did not undergo chemical decomposition to form trifluoromethyl sulfide during the exposure experiment.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1990
- Accession Number
- ADA224445
Entities
People
- Ernest H. Braue Jr.
- Michael G. Pannella
Organizations
- United States Army Medical Research Institute of Chemical Defense