Effects of Pressure on Membrane-Associated Receptors and Effector Elements
Abstract
To assess the effects of hydrostatic pressure on transmembrane signal transduction, we hace examined the A1 adenosine receptor - G protein - adenylyl cyclase complex in brain tissue of marine teleost fishes which occur at different depths. We have used two congeneric species, Sebastolobus alascanus and S. altivelis and the deeper-living Antimora rostrata. The function of adenylyl cyclase and modulation of basal adenylyl cyclase activity by A1 adenosine receptor agonists of ATP for adenylyl cyclase is pressure of 272 atm in brain membranes from A. rostrata. The K sub m of ATP for adenylyl cyclase is pressure sensitive in te Sebastolobus species. However, the adenylyl cyclase of the deeper-living S. altivelis is less affected by pressure increases. Pertussis toxin-catalyzed (32P) ADP-ribosylation of G proteins occupancy of the muscarinic cholinergic and adenosine receptors. The phospholipid and fatty acid compositions of the Sebastolobus species are similar.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 25, 1990
- Accession Number
- ADA224871
Entities
People
- Joseph F. Siebenaller
- Thomas F. Murray
Organizations
- Oregon State University