Cellular and Molecular Mechanisms of High Pressure Inotropy in Cardiac Muscle
Abstract
We have spent the past year designing, building and testing the components of a microfluorimeter system for measuring cytosolic calcium in single cardiac cells at high hydrostatic pressure. The central element of the system is a 75 microliter cell chamber fitted with windows and placed in the optical axis of a Zeiss inverted microscope. An optical network has been devised to record simultaneously the fluorescent emission from FURA-II, a calcium reporter dye, and sarcomere spacing from differential interference contrast imaging. To achieve the required microsecond time resolution and sub-micrometer space resolution we have integrated optical, mechanical and electrical components using an 80386/80387 central processor with custom interface hardware and software. Details of these technical developments are provided in our annual report. Keywords: Membranes, Cardiac muscle, Contraction force, Calcium, Inotropy, Myocyte, Pressure.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1990
- Accession Number
- ADA224944
Entities
People
- Perry M. Hogan
- Stephen R. Besch
Organizations
- University at Buffalo