Acetylcholinesterase Chirality and Cellular Mechanisms of Organophosphonate Toxicity
Abstract
These studies concern the chiral reactivity of AchE, and examine the topography of the enzyme subunit. Several independent kinetic and equilibrium indices of ligand orientation are employed: bimolecular inhibition, oxime reactivation, aging, and equilibrium dissociation constants. Organic synthesis is an integral part of these studies and we developed several families of achiral and chiral, and fluorescent and non-fluorescent organophosphonates and reversible noncovalent fluorescent probes. These agents are employed in study of AchE from Torpedo, as one means for probing subunit topography, as well as in in vivo and in vitro studies concerning cellular regulation of AchE. Keywords: Acetylcholinesterase; Organophosphates; Organophosphonates; Chirality; Cellular toxicity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 11, 1990
- Accession Number
- ADA224981
Entities
People
- Harvey A. Berman
Organizations
- University at Buffalo