Factors That Contribute to Neuron Survival and Neuron Growth after Injury
Abstract
One series of experiments has studied regeneration of the cut central processes of adult dorsal root ganglion cells into transplants of embryonic central nervous system tissue. Anatomical tracing methods show that cut dorsal roots regenerate into intraspinal transplants of embryonic spinal cord. Immunocytochemical techniques show that the regenerated dorsal roots contain calcitonin gene-related peptide (CGRP) and that many of these axons establish synapses within transplants that resemble those formed in the normal spinal cord. Cut dorsal roots also regenerate into intraspinal transplants of embryonic brain, but growth into embryonic spinal cord transplants is more robust. Another series of experiments has used immunocytochemical, in situ hybridization, and cell counting methods to show that cutting the peripheral processes of dorsal root ganglion neurons has profound effects on tachykinin synthesis and can cause the cells to die, whereas the neurons survive axotomy of their central processes and tachykinin synthesis is unaffected.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 07, 1990
- Accession Number
- ADA225294
Entities
People
- Alan Tessler