Immunological Approach to the Identification and Development of Vaccines to Various Toxins
Abstract
Monoclonal antibodies of relatively high binding affinity constants specific for the sodium channel blockers saxitoxin and tetrodotoxin, and the synthesis inhibitor mycotoxin T-2 were generated. Two monoclonal antibodies against saxitoxin and two against tetrodotoxin were isolated and shown to inhibit the binding of saxitoxin and tetrodotoxin, respectively, to rat brain cell membrane. They were also effective in protecting against the saxitoxin and tetrodotoxin-induced reduction of peripheral nerve action potentials in rat ribial nerve when administered in situ. Polyclonal rabbit and monoclonal anti- idiotypic antibodies have been generated and characterized. An IgG1k mAb specific for T-2 was also generated. This antibody, termed HD11 (IgG1), completely protected the human cell lines Hep-2 and KB against T-2-induced cytotoxicity. Keywords: Biological toxins, Saxitoxin, Tetrodotoxin, Mycotoxin, Anti-idiotypes, Vaccines, Toxicity, Protection, Monoclonal antibodies, High binding, Affinity constants.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 18, 1990
- Accession Number
- ADA225374
Entities
People
- Deborah Armstrong
- Ronald C. Kennedy
- Tran C. Chanh
Organizations
- Texas Biomedical Research Institute