Studies of Infection and Dissemination of Rift Valley Fever Virus in Mosquitoes

Abstract

We have been engaged in a multimethod study of Rift Valley fever (RVF) virus in mosquitoes. During this year, we have carried out: (1) immunocytochemical and ultrastructural studies of the proventriculus of adult, female Culex pipiens infected with RVF virus. (2) immunocytochemical studies of the salivary glands and other tissues in Anopheles sp. which had been shown to be non-transmitters when intrathoracically (IT)-infected as adults, but transmitters when IT-infected as immatures. (3) work on the development of an immunogold procedure for in situ labelling of RVF virions in electron microscopic preparations. (4) work on the application of a cDNA probe for the in situ localization of RVF viral genome in serial paraffin sections of mosquitoes. (5) work on the identification of the mosquito cell surface receptor molecule for RVF virus.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1990
Accession Number
ADA225496

Entities

People

  • William S. Romoser

Organizations

  • Ohio University

Tags

DTIC Thesaurus Topics

  • Animal Structures
  • Antibodies
  • Biomedical And Dental Materials
  • Cell Line
  • Cell Membrane
  • Cells
  • Cellular Structures
  • Electron Microscopy
  • Epithelial Cells
  • Histology
  • Medical Personnel
  • Microscopy
  • Morphogenesis
  • Polymeric Films
  • Proteins
  • Rift Valley Fever
  • Tissue Extracts

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Vector-Borne Disease and Entomology
  • Virology (or Medical Virology).

Technology Areas

  • Microelectronics