Autocrine Stimulation of Interleukin-1(alpha) and Transforming Growth Factor(alpha) Production in Human Keratinocytes and Its Antagonism by Glucocorticoids,
Abstract
Interleukin-1 (IL-1) and transforming growth factor alpha (TGF alpha) mRNA expression was analysed in cultured normal human keratinocytes. Keratinocytes constitively express IL-1 mRNA when cultured in keratinocyte growth medium but not in Dulbecco's minimal essential medium containing fetal bovine serum, in which the cells differentiate. The predominant form of IL-1 expressed by keratinocytes is IL-1 alpha. Addition of IL-1 alpha to keratinocytes increased IL-1 alpha and TGF alpha mRNA expression in a dose-dependent manner. TGF alpha induced a similar increase in IL-1 alpha and TGF alpha mRNAs in keratinocytes. Hydrocortisone decreased the expression of both IL-1 alpha and TGF alpha mRNAs in keratinocytes. These findings document an autocrine mechanism by which IL-1 alpha and TGF alpha can stimulate the proliferation of keratinocytes in the skin. Acquired immune deficiency syndrome. (js)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1989
- Accession Number
- ADA226504
Entities
People
- Antnony C. Allison
- Elsie M. Eugui
- Mariola Ilnicka
- Simon W. Lee
- Vera B. Morhenn
Organizations
- Stanford University