Quarterly Report (3rd) for Contract N00014-88-C-0755 (California University)
Abstract
Six donors have been studied using Tc-99m. One unit of blood was drawn from each donor, concentrated to red cells and stored in the blood bank refrigerator for 5 days. The unit was then glycerolized and frozen. When needed, each unit of blood was thawed and deglycerolized. Enough saline-dextrose solution was added to the cells to make a hematocrit of 45% 2%. CPDA-1 solution was then added in a ratio of 63ml. of anticoagulant to 450 ml. of blood. The red cells were then placed in the blood bank refrigerator and stored for 14 days. At the thirteenth day, a sample of blood was removed and analyzed for sterility using the Bactec instrument. The next day, an aliquot of the blood was then removed and 250 uCi. of Tc-99m was added. This labelled blood was injected into the donor, and baseline samples were drawn at 10, 15, 20 and 60 minutes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1990
- Accession Number
- ADA226506
Entities
People
- Byron A. Myhre
Organizations
- University of California, Los Angeles