Sulfur Mustard (SM) Lesions in Organ-Cultured Human Skin: Markers of Injury and Inflammatory Mediators
Abstract
The paranuclear vacuolization test for injury to human skin. Full thickness specimen of human skin (1.0 cm 2) were topically exposed to 10 yl of 0.03% to 1.0% SM and organ-cultured for 24 hr at 36 C. There was a straight-line dose-response relationship between the above concentrations of SM and the number of paranuclear vacuoles seen historically in the epidermis. Within the same SM dosage range, there was also a proportional decrease in 14 C-leucine incorporation by the explants. Early mediators of inflammation produced by SM in human skin. Culture fluids from SM-exposed and control full-thickness human skin explants contained similar amounts of angio-tensin-converting enzyme, tryspin- like and chymotryspin-like proteases, acid phosphatase, B-glucuronidase, B- galactosidase, lysozyme, deoxyribonuclease, ribonuclease, interleukin 1 and lactic dehydrogenase. However, the culture fluids from the SM-exposed explants contained increased amounts of histamine, plasminogen activator (PA), and usually, prostaglandin E2 (PGE2), compared to culture fluids from control explants. Keywords: Sulfur mustard, full-thickness human skin explants in organ culture; histology & histochemistry; Inflammatory medidators; Viability of human skin at 4 C.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 16, 1990
- Accession Number
- ADA227358
Entities
People
- Arthur M. Dannenberg Jr.
Organizations
- Johns Hopkins University