Molecular Mechanisms of Cytopathogenicity of Primate Lymphotropic Retroviruses: Relevance to Treatment and Vaccine for Aids
Abstract
Studies were designed to examine the molecular basis of infectivity, cytopathogenicity and genomic activation of HIV-1, with the particular emphasis on examining the factors relevant to the design of treatment and vaccine programs for combating HIV-1 infection. Three parallel approaches were followed in the course of this work. The results of this work can be summarized as follows: (1) In order to obtain a better understanding of viral gene function involved in cell killing, a series of HIV-1 viral deletion mutants were generated and the biological consequences of these mulations were examined by transfection into recipient host cells. Our results have shown that mutations in vif result in the production of morphologically normal virus particles, but which are deficient in cell-free (but not cell-to-cell) transmission to permissive cells. Mutations in the carboxy region of gp41 result in dramatic changes in the cell-to-cell transmission and cytopathic properties of the virus. (js)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 10, 1990
- Accession Number
- ADA227693
Entities
People
- Linda L. Jagodzinski
- Mark M. Manak