Smooth Muscle Model System for the Induction of Oxygen Regulated Proteins during Ischemia
Abstract
In this report, proliferating and differentiated myogenic cells have been used to evaluate the effects of hypoxia on the cell cycle distribution and protein synthesis in vascular smooth muscle. The data demonstrate potentiation of myocyte responsiveness to growth stimuli by hypoxia. The modulation of phenotype was also observed as reduced glucose consumption and lactate production rates. Although the induction of GRPs and ORPs in myogenic cells was independent of their state of differentiation; suggesting that these are generic responses to ischemic stress; differences in protein synthesis were observed. Hypoxia generally induced or enhanced protein synthesis in myocytes but primarily inhibited protein synthesis in myoblasts. Moreover, two proteins (PSP100 and PSP9) were identified among those induced in myocytes by hypoxia, which were otherwise characteristic of the myoblast phenotype. (js)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 30, 1990
- Accession Number
- ADA227993
Entities
People
- Arthur R. Strauch
- Harold C. Smith
Organizations
- University of Rochester