Development of an Assay to Detect Antibodies to HIV-2 Using Recombinant DNA Derived Antigens

Abstract

To develop an EIA capable of detecting HIV-2 seropositive individual s, we cloned and expressed contiguous regions of the HIV-2 envelope gene. One region that we call K1, when expressed in E. coli, produces a 36 KD protein that reacts with all seropositive samples tested, indicating that is (K1) contains the immunodominant epitopes of the envelope protein. Other regions show little or no reactivity with seropositive samples. We have developed procedures for large scale purification of the K1 protein and have detection of HIV-2 seropositive individuals. (js)

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Document Details

Document Type
Technical Report
Publication Date
Sep 11, 1990
Accession Number
ADA228158

Entities

People

  • Gerald Beltz

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amides
  • Amino Acids
  • Anhydrides
  • Antibodies
  • Biomedical And Dental Materials
  • Cells
  • Cellular Structures
  • Chemistry
  • Detection
  • Genetic Structures
  • Laboratory Animals
  • Materials
  • Molecular Weight
  • Proteins
  • Recombinant Dna
  • Security
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and Cellular Biochemistry