Genetic and Physiological Studies of Bacillus Anthracis Related to Development of an Improved Vaccine

Abstract

Physical analysis of the Bacillus anthracis toxin plasmid, pX01.1, has confirmed the regions of PX01.1 involved in toxin production and further defined the regions surrounding the toxin genes. DNA-DNA hybridzation analysis confirmed that the restriction fragments of pX01.1 which contain the toxin structural genes correspond to similar fragments on the restriction map of pX01 prepared by D. Roberston. DNA-DNA hybridization analysis of the Tn917-tagged deletion derivative from Weybridge A UM23 tp21, which has the interesting phenotype Pa-LF+Ef+, showed that the 34.8-kb fragments together to form a new 44.2-kb BamHi fragment. The results obtained from analysis of the plasmid from UM23 tp21 are consistent with the PA-LF+Ef+ phenotype observed in this mutant.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1990
Accession Number
ADA228328

Entities

People

  • Curtis B. Thorne

Organizations

  • University of Massachusetts Amherst

Tags

DTIC Thesaurus Topics

  • Acquisition
  • Anti-Bacterial Agents
  • Bacteria
  • Bacteriophages
  • Biological Toxins
  • Cells
  • Coatings
  • Culture Media
  • Genetics
  • Identification
  • Medical Personnel
  • Microbiology
  • Molecules
  • Phosphodiesterases
  • Plastic Explosives
  • Precipitation
  • Transfer Functions

Readers

  • Microbial Pathology
  • Molecular Genetics

Technology Areas

  • Biotechnology