Visual Enhancement of Myelinated Tissues in the Central Nervous System of the Rat Using Sudan Black B

Abstract

This report investigated the quantitative distribution of radio labelled compounds in the central nervous system of the rate using autoradiographic techniques. To complement our autoradiographic images, we searched for a suitable fiber stain delineating myelinated structures using fresh frozen tissue. Surprisingly, the majority of staining procedures were exclusive to fixed tissues. These techniques applied to fresh frozen brain tissue produced poor differentiation of myelinated and nonmyelinated areas and were generally unsatisfactory. Therefore, we preformed a series of studies using lipid soluble dyes such as oil red O, Luxol fast blue, hematoxylin and Nile blue in an effort to enhance differentiation of myelin. We found Sudan black B to be superior to enhance differentiation of myelin. We found Sudan black B to be superior for our purpose.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1990
Accession Number
ADA228461

Entities

People

  • Karen R. Olson
  • Richard K. Traub

Organizations

  • United States Army Medical Research Institute of Chemical Defense

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Anatomy
  • Biological Sciences
  • Biological Staining And Labeling
  • Biomedical Research
  • Boiling Point
  • Brain
  • Cell Nucleus
  • Central Nervous System
  • Choroid Plexus
  • Classification
  • Dyes
  • Indicator Dyes
  • Nervous System
  • Security
  • Tissues
  • Water

Readers

  • International Relations and Conflict Resolution
  • Neuroscience
  • Oncology and Biomarker-Based Cancer Detection.