Pathophysiology and Toxicokinetic Studies of Blue-Green Algae Intoxication in the Swine Model

Abstract

Nonlabelled and tritium labelled dihydromicrocystin-LR (2H-MCLR) were produced by reacting MCLR with sodium borohydride and 3H-sodium borohydride, respectively. Chemical purity in excess of 99% and radiochemical purity of over 98% were demonstrated with TLC, HPLC, and mass spectrometry. The labelled toxin was stable for two wk in ethanol at -20C and significant, biological instability of the label was not apparent as indicated by the presence of tritiated water in urine of dosed animals. Keywords: Blue-green algae, Phycotoxin, Microcystin, Cyanoginosin, Anatoxin-a(s), Pathophysiology, Radiolabelled toxin, Fate, Distribution, Elimination.

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Document Details

Document Type
Technical Report
Publication Date
Aug 13, 1990
Accession Number
ADA228597

Entities

People

  • Andrew M. Dahlem
  • Val R. Beasley
  • Wanda M. Haschek-hock
  • Wayne M. Carmichael
  • William O. Cook

Organizations

  • University of Illinois Urbana–Champaign

Tags

DTIC Thesaurus Topics

  • Analysis Of Variance
  • Animal Structures
  • Arteries
  • Biomedical Research
  • Blood
  • Cardiovascular Physiological Phenomena
  • Cardiovascular System
  • Cells
  • Chemistry
  • Cyanobacteria
  • Enzyme Inhibitors
  • Laboratory Animals
  • Nervous System
  • Peripheral Nervous System
  • Small Intestine
  • Statistical Analysis
  • Veins

Readers

  • Analytical Chemistry
  • Chemistry (specifically Chemical Fluorescence)
  • Toxicology/Environmental Toxicology