Effect(s) of Pharmacologic Intervention on Oxygenation, Lung Water and Protein Leak in the Pseudomonas ARDS Porcine Model
Abstract
Delayed treatment with ibuprofen in this porcine model of acute lung injury confers significant protection from the lung injury induced by Pseudomonas infusion. Delayed treatment dampened the neutrophil respiratory burst and caused a significant reduction in plasma TNF levels. Immunofluorescent flow cytometry studies demonstrate that the neutropenia in this model is associated with a significant increase in the neutrophil CD18 adhesion receptor expression by circulating neutrophils and this coincides with significant increases in plasma TNF activity. In vitro studies incubating porcine neutrophils with TNF demonstrate a significant increase in CD18 expression in vitro. An in situ perfusion technique has been developed for recruitment of pulmonary intravascular macrophages. Keywords: Lab animals; Acute respiratory distress; Pigs; Capillary permeability; Swine.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1990
- Accession Number
- ADA228866
Entities
People
- A. A. Fowler
- C. J. Walsh
- Harvey J. Sugerman
- P. D. Carey
Organizations
- Virginia Commonwealth University School of Medicine