Role of Second Messengers in Ischemic Tissue Damage
Abstract
The aim of this project is to define the second messengers responsible for the regulation of microvascular endothelial cell structure and function and to determine the mechanisms by which ischemia and reperfusion alters these messengers to produce injury to the skin. This report reviews results obtained in the following areas: A. cyclic AMP, calcium, and protein kinase C in the regulation of neovascularization under normal and ischemic growth conditions; B. the mechanisms by which protein kinase C, calcium, and vimentin are coordinated to regulate microvascular endothelial cell shape and function; C. molecular mechanisms responsible for regulation of endothelial cell shape and function; C. molecular mechanisms responsible for regulation of endothelial cell structure; D role of neutrophils and ischemia in producing endothelial cell damage; and E. dexamethasone and lipopolysaccharide in neutrophil damage to microvascular endothlial cells. The techniques that were used in this project phase contrast and electron microscopy, digital imaging fluorescence microscopy, immunocytochemistry to detect changes in the cytoskeletal proteins; and northern blot analysis for the determination of protooncogene activation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1990
- Accession Number
- ADA228868
Entities
People
- Marvin A. Karasek
Organizations
- Stanford University