Structure and Expression of Genes for Flavivirus Immunogens
Abstract
Progress during the report period included: (1) Extension of the nucleotide sequence analysis of the Japanese encephalitis (JE) and dengue serotype 1 (DEN-1) genetic material; (2) expressed in E. coli both fusion and non-fusion derivatives of the envelope structural glycoprotein (E) and the non- fusion derivatives of the envelope structural glycoprotein (E) and the nonstructural glycoprotein NS1; (3) used deletion analysis to define the minimal amino acid sequences necessary for the binding of anti-JE and anti-DEN-1 monoclonal antibodies; (4) determined that fusion derivatives of JE E and NS1 proteins expressed in E. coli do not provide protective immunity in mice; (5) developed nonradioactive nucleic hybridization probes for sensitive detection of DEN RNAs in virus-infected cells; (6) initiated collaborative efforts to express JE and DEN-1 antigens in baculovirus and vaccinia virus expressions systems; (7) established a culture facility for the growth and analysis of DEN-1. (JS)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 28, 1990
- Accession Number
- ADA229651
Entities
People
- Maurille J. Fournier
- Thomas L. Mason
Organizations
- University of Massachusetts Amherst