Subcellular Signal Transduction Systems in the Cellular Trauma of Ischemia
Abstract
The formation of sphingosine,a potent inhibitor of protein kinase C, has been characterized in cell models of interest to studies of hypoxic and ischemic injury. With J775 macrophages, we find that sphingosine is released in an acidic compartment and is inhibitable by ammonium ion, an agent that is well known to accumulate during ischemia. The increase in sphingosine is correlated with inhibition of protein kinase C; therefore, these findings may provide a link between changes in ammonia in vivo and alteration in cell function.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 24, 1990
- Accession Number
- ADA229876
Entities
People
- Alfred H. Merrill Jr
Organizations
- Emory University