Cutaneous Toxicity of Mustard and Lewisite on the Isolated Perfused Porcine Skin Flap
Abstract
This report describes the utilization of the isolated perfused porcine skip flap (IPPSF) to characterize the cutaneous toxicity of sulfur mustard (XHD) and lewisite (XL). The IPPSF is an humane, alternative, in vitro, animal model consisting of an isolated perfused skin preparation with intact and viable epidermis and dermis in association with a functional microcirculation. Task 1 of the present contract was to characterize the dose-response profile of both vesicants in 53 IPPSFs using biochemical, physiological, and morphological (light and transmission electron microscopy) endpoints. Twenty-four IPPSFs were treated with 200 microliters of either 5.0, 2.5, 1.25, 0.2, 0.14, or 0.07 mg/ml XL, and 29 IPPSFs were treated with 200 micron l of either 10.0, 5.0, 2.5, 1.25, 0.5 or 0.2 mg/ml XHD. At least four replicates were performed per compound, and four untreated IPPSFs served as controls. Experiments lasted 8 hours. Both XL and XHD resulted in a reduction in the cumulative glucose utilization and an increased vascular resistance as compared to controls. The high-dose groups for both agents consistently showed a marked alteration in both parameters.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1990
- Accession Number
- ADA229922
Entities
People
- Jim E. Riviere
- John R. King
- Nancy A. Monteiro-riviere
Organizations
- North Carolina State University