Binding of New Methylene Blue to Endotoxins and Its Effects on the Endotoxin Activity Studied By Double Diffusion and Limulus Amebocyte Lysate Assays
Abstract
New methylene blue was found to bind to smooth and rough forms of endotoxins and their components lipid A and lipid X in agarose double diffusion experiments. It also bound to dextran sulfate, heparin, transfer RNA, succinylated bovine serum albumin, trypsin, pepsin and casein, but not bovine serum albumin. Charge-charge interactions between the dye and these molecules to the formation of dye-endotoxin complex. Binding of the dye to endotoxin was found to block the endotoxin function in both gelation and chromogenic limulus amebocyte lysate assays. The potency of the dye in such inhibition was comparable to that of polymyxin B.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 30, 1989
- Accession Number
- ADA230209
Entities
People
- Akindele O. Johnson
- Che-hung Lee
Organizations
- Naval Medical Research Center