Characterization of Ground Squirrel Retinal Ganglion Cells

Abstract

Our work during this period mainly involved setting up new electrophysiological equipment for intracellular injections of retinal ganglion cells and performing immunohistochemical studies on the ground squirrel retina. We completed experiments relating to the nature of neurotransmitters/modulators in this retina, with particular interest in the ganglion cell layer. We investigated (1) the distribution of GABAergic neurons using antibodies against GABA and GAD and (2) the distribution of catecholamines using an antibody against tyrosine hydroxylase (TH). Retinal sections and wholemounts were incubated with the particular antibody and then processed using immunofluorescence and/or the avidin-biotin method.

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Document Details

Document Type
Technical Report
Publication Date
Dec 31, 1990
Accession Number
ADA230311

Entities

People

  • Ivonne Santiago
  • N. Lugo-garcia
  • R. E. Blanco

Organizations

  • University of Puerto Rico

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biological Sciences
  • Catecholamines
  • Cells
  • Chemical Compounds
  • Interdisciplinary Science
  • Laser Dyes
  • Microspheres
  • Modulators
  • Neurology
  • Neurons
  • Neurophysiology
  • Neurosciences
  • Rodents
  • Tyrosine

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Neuroscience