Liquid Collagen Wound Coverings
Abstract
We have made some changes in the collagen preparation since our last report. These changes are primarily directed at more rigorous aseptic technique. This includes the use of static-hoods and U.V. steri-lamps which have been adapted to the laboratory preparation of collagen. This serves to reduce the physical burden on membrane filtration. In addition, we have added an initial defatting step prior to further processing of the fetal calf skin. This better ensures the production of pure final product of Type I collagen. Defatting of the skin also enables more efficient filtration of the collagen. An additional concern was the adverse effect iodine may have on wound healing, even though it is used at a very low concentration in our preparation. Iodine has proven to be a very efficient means of cross-linking the collagen and sterilizing the wound bed, making its presence at the initial stages of therapy desirable. However, the persistence of iodine during the fragile re-epithelization process could prove to be detrimental. We devised a means of neutralizing the deleterious effects of iodine by converting it to an inert salt. (js)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 10, 1990
- Accession Number
- ADA230390
Entities
Organizations
- Oregon Health & Science University