Genetic Engineering of Clostridium Difficile Toxin a Vaccine

Abstract

The long range goal of this project is to use C. difficile toxin A as a model to add technical information in the area of vaccine biotechnology using recombinant DNA techniques. This will be accomplished in part by predicting epitopes from the DNA sequence of toxin A, synthesizing DNA for these predicted regions, and testing their antigenic activity in expression vectors. Part of the toxin A gene has been cloned and sequenced. A total of twelve different epitodes have been predicted, synthesized, cloned into lambda gtll and tested for antigenicity. Alternative procedures for epitode mapping has been described and initiated.

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Document Details

Document Type
Technical Report
Publication Date
Aug 16, 1990
Accession Number
ADA230411

Entities

People

  • Joe Johnson
  • Lycurgus L. Muldrow

Organizations

  • Spelman College

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antibodies
  • Biotechnology
  • Chemistry
  • Classification
  • Clones
  • Clostridium
  • Dna Sequence Analysis
  • Genetic Engineering
  • Genetic Structures
  • Hybridization
  • Immune Serums
  • Liquid Chromatography
  • Materials
  • Oligomers
  • Recombinant Dna
  • Security
  • Vaccines

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular Genetics
  • Software Engineering

Technology Areas

  • Biotechnology