Rescue of Injured Myocytes
Abstract
The crucial events leading to loss of cell viability after hypoxic and toxic injury remain poorly understood. Cell death is rarely synchronous, and rapidly occurring events leading to irreversible injury may be obscured when large cell populations are studied. To overcome this drawback, single cultured hepatocytes and myocytes during hypoxic and toxic stress were studied using a new, rapidly developing technology called Multiparameter Digitized Video Microscopy (MFBM). In MDVM, living cells are labeled with multiple fluorescent probes. Each probe responds to a defined cellular parameter by emitting fluorescence of a specific wavelength. 2-dimensional images of the fluorescence of each probe are digitized and stored during the course of cell injury. In this way, several cellular parameters are monitored quantitatively over time.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 30, 1990
- Accession Number
- ADA230505
Entities
People
- John J. Lemasters
Organizations
- University of North Carolina at Chapel Hill