Analysis of Dengue Virus Enhancing Epitopes Using Peptide Antigens Derived from the Envelope Glycoprotein Gene Sequence
Abstract
Antibody-dependent enhancement (ADE) of dengue (DEN) virus infection in human mononuclear cells in vitro has been standardized using a human promonocytic cell line HL-CZ, purified monoclonal antibodies (MAbs), and select DEN viruses. Characterization of the FC-receptors (FcRs) expressed on HL-CZ cells have indicated that subsets of FcR mediate ADE better than others. Using this standardized system, we have compared the ability of mouse anti-DEN 2 envelope (E) peptides to elicit virus neutralization and ADE. Peptides 1-2, 437 appear to elicit ADE activity in contrast to other peptides that appear to elicit neutralization but not ADE. Though these assays need to be repeated, it appears that differential functions may be attributed to particular E genomic regions. The comparison of the nucleotide sequences of Den-1 RNA encoding the non-structural proteins to the other DEN sequences has revealed that DEN-4 and Den-1 share > 90% similarity in NS3 and NS4a, 4b genome regions. DEN-3 and DEN-1 have a deletion in NS5 that is conserved in other DEN-1 and DEN-3 isolates. These genomic sequence comparisons indicate that non-structural region differences need to be studied as well for our understanding of DEN replication and pathogenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 27, 1990
- Accession Number
- ADA230976
Entities
People
- May C. Chu
Organizations
- Centers for Disease Control and Prevention