Determination of Dimethyl Sulfoxide (DMSO), Ethanol (ETOH), Formamide (F) and Glycerol/Formal (GF) by High Performance Liquid Chromatography (HPLC)

Abstract

Liquid chromatographic (LC) methods have been developed for the determination of dimethyl sulfoxide (DMSO), ethanol (ETOH), formamide (F), and glycerol/formal (GF), in aqueous samples. The samples are directly injected onto a high performance liquid chromatograph (HPLC) without further sample preparation. Separation of DMSO and F was achieved by using a C18 column and a mobile phase containing water/methanol. Separation of GS and ETOH was achieved by using a Dextro-Pak column and a mobile phase consisting of water. A programmable ultraviolet (UV) spectrophotometric detector was used to monitor the LC effluent for DMSO and F. A refractive index detector was used to monitor the LC effluent for ETOH and GF. The precision and accuracy of all methods are included in the report.

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Document Details

Document Type
Technical Report
Publication Date
Jan 30, 1989
Accession Number
ADA231385

Entities

People

  • Alan B. Rosencrance
  • Ernst E. Brueggemann

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Accuracy
  • Alcohols
  • Biomedical Research
  • Chromatographs
  • Chromatography
  • Classification
  • Detection
  • Detectors
  • Health Services
  • Instrumentation
  • Liquid Chromatography
  • Methanols
  • Physical Properties
  • Precision
  • Refractive Index
  • Security
  • Standards

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry