Detection of Microbial Trypsin-Like Enzymes by Use of an Agar Gel

Abstract

Certain oral micro-organisms associated with periodontal diseases elaborate trypsin-like enzymes. This study describes a simple assay for these enzymes, based on the incorporation of the synthetic substrate N-Alpha-benzoyl- DL-arginine-p-nitroanilide (BAPNA) into ion-agar gel. The system can provide quantitative or qualitative data within 24 h and is suitable for use in the dental clinic.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1990
Accession Number
ADA231709

Entities

People

  • B. L. Lamberts
  • E. D. Pederson

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Actinobacteria
  • Bacteria
  • Bacterial Infections
  • Bacteroidaceae
  • Biomedical Research
  • Culture Media
  • Detection
  • Diseases And Disorders
  • Filter Paper
  • Gram-Negative Bacteria
  • Great Lakes
  • Laboratory Procedures
  • Mouth Diseases
  • Navy
  • Substrates
  • United States

Fields of Study

  • Biology

Readers

  • Aerial Unmanned Vehicle Swarm Micro Periodontal Dentistry.
  • Computational Modeling and Simulation
  • Microbial Pathology

Technology Areas

  • Biotechnology