Workshop on Biophysics of Transmembrane Electric Fields

Abstract

It is common accepted that efficiency of cell electrofusion is higher when cells are preliminary brought in closer contact. On this base two new approaches in cell electrofusion have been developed. The first one, fusion of cells on an electroconductive (cellulose) film, is very effective for spreading cells (e.g. mouse L-cells) that are able to attach to solid surfaces. The second, more universal, approach is electrofusion of cells in centrifugated pellets. Specific features of cell contacts and possible mechanism of electrofusion are discussed. Electric fields are supposed to initiate fusion process only in areas where plasma membranes of contacting cells are divided narrow extended gaps.

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Document Details

Document Type
Technical Report
Publication Date
Nov 15, 1990
Accession Number
ADA232057

Entities

People

  • Arthur E. Sowers

Tags

Communities of Interest

  • Biomedical
  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Amino Acids
  • Biophysics
  • Cell Membrane
  • Cell Physiological Processes
  • Cells
  • Chemical Analysis
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Confocal Microscopy
  • Electric Fields
  • Electrical Properties
  • Electromagnetic Fields
  • Fish
  • Membrane Lipids
  • Molecular Dynamics
  • Rodents

Readers

  • Molecular and Cellular Biochemistry
  • Solar Photovoltaics and Thermoelectric Devices.
  • Systems Analysis and Design

Technology Areas

  • Biotechnology