Isolation, Cloning and Expression of the Genes for Microbial Polyurethane Degradation
Abstract
New degradation tests are in progress to determine if polyurethane (Impranil-R) and/or urethane (Sigma) can be used as sole carbon source. Fifteen different cultures have been inoculated into 10 ml polyurethane- and urethane- minimal salts solutions. The DNA vector and fungal host, received from the Fungal Genetics Stock Center, Department of Microbiology, University of Kansas Medical Center are cosmid pSV50 in an E. coli host, LM83, and Neurospora crassa wild type strain (74-OR23-1VA), FGSC 2489. The genomic library will be constructed in E. coli HB101. The fungal vector host will be used for expression of the genes from HAFB-2F-Br. While the DNA isolation procedure is being optimized, concurrent experiments will be done to optimize the transformation frequency of both the E. coli competent cells and the Neurospora crassa host using previously published procedures.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 20, 1991
- Accession Number
- ADA232108
Entities
People
- Gail L. Bowers-irons
- Ramesh K. Prakash
- Robert Pryor
- Usha Charyulu