Plasma Catecholamine Degradation with Long-Term Storage

Abstract

The stability of norepinephrine (NE) and epinephrine (EPI) during long-term storage at -70 C was analyzed in plasma from humans, canines, and rats. In human plasma, heparin and EDTA as anticoagulants, in combination with reduced glutathione and EGTA (GSH/EGTA) as a preservative, were compared. Canine plasma was heparinized and contained GSH/EGTA as a preservative. Rat plasma was heparinized without the addition of preservative. Norepinephrine and EPI levels were measured by high pressure liquid chromatography (HPLC) with electrochemical detection. Optimal storage conditions for NE and EPI in human plasma require heparin as an anticoagulant without the addition of GSH/EGTA as a preservative. Norepinephrine and EPI in human plasma were stable for approximately 18 months (550 days).

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Document Details

Document Type
Technical Report
Publication Date
Jan 22, 1991
Accession Number
ADA233125

Entities

People

  • H. L. Reed
  • M. J. Malik
  • M. M. D'alesandro

Organizations

  • Naval Medical Research Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Aluminum Oxides
  • Animals
  • Anticoagulants
  • Biomedical Research
  • Blood
  • Catecholamines
  • Degradation
  • Epinephrine
  • High Pressure
  • Laboratory Animals
  • Linear Regression Analysis
  • Liquid Chromatography
  • Materials
  • Norepinephrine
  • Regression Analysis
  • Security
  • Veins

Readers

  • Cardiovascular Physiology
  • Materials Science and Engineering.
  • Molecular and Cellular Biochemistry