In Vitro Growth Inhibition of Plasmodium Falciparum by Sera from Different Regions of the Philippines

Abstract

Sera from different malaria endemic regions of the Republic of the Philippines were compared for their ability to inhibit growth of Plasmodium falciparum in vitro. Dialyzed serum was added to synchronous cultures containing schizonts for either the total 48 hr test period or only the last 24 hr in order to analyze the effects on erythrocytic invasion and intraerythrocytic growth, respectively. Reduction in 3H-hypoxanthine uptake was used to determine the percent inhibition compared to nonimmune serum. One hundred seventy sera from Mindanao and Palawan in South, the centrally located island of Mindoro, and Luzon in the North, were tested against 4 P. falciparum strains from the Philippines and 1 from Africa. Indirect fluorescent antibody titers were not predictive of inhibition. Inhibition of merozoite invasion rather than intraerythrocytic parasite growth is suggested by this study.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1990
Accession Number
ADA233226

Entities

People

  • N. W. Sy
  • P. S. Macalagay
  • R. B. Oberst
  • S. F. Cruzada
  • V. D. Fallarme

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Antibodies
  • Biomedical Research
  • California
  • Immune Serums
  • Immunoglobulins
  • Inhibition
  • Islands
  • Malaria
  • Medical Personnel
  • Navy
  • Parasites
  • Philippines
  • Republic
  • Spores
  • Tropical Medicine
  • United States

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • International Relations, focusing on Korea-Africa and North Korea-South Korea relations, and Nigeria-Latin American Relations.
  • Parasitology and Pharmacology of Malaria.